Further studies on the mode of action of the heme-controlled translational inhibitor: stimulating protein acts at level of binary complex formation. | Zendy

C de Haro | Zendy; S Ochoa | Zendy

Open Access

Further studies on the mode of action of the heme-controlled translational inhibitor: stimulating protein acts at level of binary complex formation.

Author(s) -

C de Haro,

S Ochoa

Publication year - 1979

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.76.5.2163

Subject(s) - ternary complex , gtp' , chemistry , reticulocyte , hemin , heme , biochemistry , biology , microbiology and biotechnology , enzyme , messenger rna , gene

Previous work has shown that (i) at physiological concentrations of eukaryotic initiation factor 2 (eIF-2), formation of the ternary complex eIF-2-GTP-Met-tRNAi, which precedes the assembly of a 40S initiation complex, requires the presence of eIF-2 stimulating protein (ESP) and (ii) the interaction of eIF-2 with ESP is blocked by the translational inhibitor which, in reticulocyte lysates, is activated in the absence of hemin. Present evidence indicates that formation of the ternary complex is preceded by formation of the binary complex eIF-2-GTP and that ESP acts at the level of binary complex formation.

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