Biochemical and electron microscopic evidence that cell nucleus negatively controls mitochondrial genomic activity in early sea urchin development. | Zendy

Annamaria Rinaldi | Zendy; Giacomo De Leo | Zendy; A. Arzone | Zendy; I. Salcher | Zendy; A Storace | Zendy; V. Mutolo | Zendy

Open Access

Biochemical and electron microscopic evidence that cell nucleus negatively controls mitochondrial genomic activity in early sea urchin development.

Author(s) -

Annamaria Rinaldi,

Giacomo De Leo,

A. Arzone,

I. Salcher,

A Storace,

V. Mutolo

Publication year - 1979

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.011

H-Index - 771

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.76.4.1916

Subject(s) - mitochondrial dna , mitochondrion , sea urchin , nucleus , biology , microbiology and biotechnology , population , cell nucleus , nuclear dna , dna , cell , biochemistry , gene , demography , sociology

Enucleated halves of sea urchin eggs obtained by centrifugation contain almost all the mitochondrial population of the egg. Removal of the nucleus followed by parthenogenetic activation stimulates the incorporation of [3H]thymidine into the mitochondrial DNA, whereas no such incorportion is observed in activated whole eggs. The block is not the result of a modification in the permeability of the mitochondrial membrane. Electron microscopic observations demonstrated duplication of mitochondrial DNA molecules in activated enucleated halves. No duplication was found in the mitochondrial DNA from activated whole eggs or from nonactivated enucleated halves. We conclude that the cell nucleus exerts a negative control on the activity of the mitochondrial genome through some short-lived nuclear substance(s).

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