Role of methylation in the modification and restriction of chloroplast DNA in Chlamydomonas. | Zendy

William G. Burton | Zendy; Constance T. Grabowy | Zendy; Ruth Sager | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Role of methylation in the modification and restriction of chloroplast DNA in Chlamydomonas.

Author(s) -

William G. Burton,

Constance T. Grabowy,

Ruth Sager

Publication year - 1979

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.011

H-Index - 771

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.76.3.1390

Subject(s) - chloroplast dna , biology , zygote , uracil , thymine , dna , chlamydomonas , genetics , dna methylation , chloroplast , restriction enzyme , cytosine , 5 methylcytosine , dna replication , biochemistry , microbiology and biotechnology , gene , mutant , embryogenesis , gene expression

The different metabolic paths followed by homologous chloroplast DNAs of maternal and paternal origins in zygotes of Chlamydomonas were examined by prelabeling parental cells, before mating them, with [3H]adenine, [3H]thymidine, and [3H]deoxycytidine. Within 6 hr after mating, maternal chloroplast DNA was extensively methylated to 5-methylcytosine and its bouyant density decreased. Paternal chloroplast DNA was largely degraded. Some radioactivity from deoxycytidine of maternal origin reappeared in thymine, and residual paternal DNA contained radioactivity in a base tentatively identified as uracil. These results confirm and extend our previous findings and support our hypothesis that modification (methylation) and restriction enzymes determine maternal inheritance of chloroplast DNA and that the two parental DNAs have different metabolic fates within the zygote.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research