Aflatoxin B1 mutagenesis, DNA binding, and adduct formation in Salmonella typhimurium. | Zendy

A A Stark | Zendy; John M. Essigmann | Zendy; A.L. Demain | Zendy; T R Skopek | Zendy; Gerald N. Wogan | Zendy

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Aflatoxin B1 mutagenesis, DNA binding, and adduct formation in Salmonella typhimurium.

Author(s) -

A A Stark,

John M. Essigmann,

A.L. Demain,

T R Skopek,

Gerald N. Wogan

Publication year - 1979

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.011

H-Index - 771

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.76.3.1343

Subject(s) - aflatoxin , mutagenesis , dna , adduct , salmonella , strain (injury) , chemistry , dna adduct , microbiology and biotechnology , escherichia coli , biochemistry , mutation , biology , bacteria , gene , genetics , food science , organic chemistry , anatomy

Salmonella typhimurium strain TM677 was mutagenized with aflatoxin B1 (AFB1) in liquid suspension culture in the presence of a rat liver postmitochondrial supernatant. Forward mutation to 8-azaguanine resistance was measured in the treated cultures and was found to increase linearly with AFB1 concentration. DNA purified from mutagenized cells was analyzed for AFB1 adduct formation by high-pressure liquid chromatography after adduct liberation. AFB1 exposures at 0.16 and 0.32 micrometer for 35 min produced 15 and 22 AFB1--DNA adducts per genome, respectively, and induced 8-azaguanine-resistant fractions of 4.9 X 10(-4) and 9.6 X 10(-4). Approximately 70% of the AFB1 bound to DNA was chromatographically identical to 2,3-dihydro-2-(N7-guanyl)-3-hydroxyaflatoxin B1 at the two AFB1 levels used.

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