Human leukocyte interferon: production, purification to homogeneity, and initial characterization. | Zendy

Menachem Rubinstein | Zendy; Sara Rubinstein | Zendy; P C Familletti | Zendy; Robert S. Miller | Zendy; Alan A. Waldman | Zendy; Sidney Pestka | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Human leukocyte interferon: production, purification to homogeneity, and initial characterization.

Author(s) -

Menachem Rubinstein,

Sara Rubinstein,

P C Familletti,

Robert S. Miller,

Alan A. Waldman,

Sidney Pestka

Publication year - 1979

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.011

H-Index - 771

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.76.2.640

Subject(s) - chromatography , chemistry , homogeneous , sodium dodecyl sulfate , homogeneity (statistics) , polyacrylamide gel electrophoresis , gel electrophoresis , electrophoresis , interferon , gel permeation chromatography , biochemistry , biology , enzyme , organic chemistry , immunology , polymer , statistics , physics , mathematics , thermodynamics

A method of fractionating proteins by high-performance liquid partition chromatography has been developed and used for isolation and purification to homogeneity of one of the species of human leukocyte interferon. The homogeneous interferon exhibited a sharp peak on high-performance liquid chromatography and a single narrow band on sodium dodecyl sulfate/polyacrylamide gel electrophoresis in the presence of 2-mercaptoethanol. Extraction of the gel gave a single sharp peak of antiviral activity coinciding with the protein band. The specific activity of pure interferon was found to be 2--4 X 10(8) units/mg, based on amino acid analysis. The molecular weight is 17,500--18,000.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research