Open AccessGene for the RNA polymerase sigma subunit mapped in Salmonella typhimurium and Escherichia coli by cloning and deletion.
Author(s) -
John Scaife,
Joseph S. Heilig,
Lee Rowen,
Richard Calendar
Publication year - 1979
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.76.12.6510
Subject(s) - primase , dnag , biology , microbiology and biotechnology , rna polymerase , escherichia coli , polymerase , gene , genetics , molecular cloning , cloning (programming) , restriction map , plasmid , polymerase chain reaction , circular bacterial chromosome , peptide sequence , reverse transcriptase , computer science , programming language
The genes for the RNA polymerase sigma subunit (rpoD) and DNA primase (dnaG) of Salmonella typhimurium have been cloned into lambda vectors. Combined restriction, deletion and functional analysis of the cloned fragment allows us to map the genes precisely on the fragment, establishes the direction in which rpoD is transcribed, and reveals the existence of at least one new gene in the vicinity. A closely homologous, smaller fragment of Escherichia coli DNA, also cloned into lambda, contains rpoD and at least part of dnaG.