Open AccessAnisotropic molecular motion on cell surfaces.
Author(s) -
Barton A. Smith,
William R. Clark,
Harden M. McConnell
Publication year - 1979
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.76.11.5641
Subject(s) - photobleaching , anisotropy , anisotropic diffusion , perpendicular , diffusion , fluorescence recovery after photobleaching , materials science , fourier analysis , biophysics , fourier transform , motion (physics) , optics , physics , chemistry , molecular physics , classical mechanics , geometry , biology , fluorescence , mathematics , quantum mechanics , thermodynamics
Photobleaching in a two-dimensional periodic pattern has been used to characterize the motion of succinylconcanavalin A receptors on the surfaces of mouse fibroblasts. These receptors were found to exhibit anisotropic two-dimensional diffusion in adherent cells having parallel stress fibers. Diffusion coefficients of the order of 10(-11) cm2/sec and 10(-12) cm2/sec were measured for diffusive motions parallel and perpendicular to these fibers. The determination of the anisotropic motion used a two-dimensional Fourier analysis. A study was made of the effect of changes in cell shape on the photobleach pattern.