Open AccessDetection of proteins like human gamma and beta globins in Escherichia coli carrying recombinant DNA plasmids.
Author(s) -
Lois B. Wilson,
H E Grenett,
D M Doran,
F A Garver,
John T. Wilson
Publication year - 1979
Publication title -
proceedings of the national academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.76.11.5631
Subject(s) - recombinant dna , escherichia coli , globin , microbiology and biotechnology , plasmid , hemoglobin , biology , complementary dna , antibody , beta (programming language) , dna , biochemistry , gene , genetics , programming language , computer science
Escherichia coli strain chi 1776 carrying recombinant DNA plasmids containing cDNA copies of human beta or gamma globin mRNAs has been shown by radioimmunoassay to synthesize polypeptides antigenically related to the beta and gamma chains of human hemoglobin. The gamma and beta polypeptides have been enriched from lysates on immunoabsorbent columns containing hemoglobin antibodies and shown to specifically inhibit the antigen-antibody binding between 125I-labeled hemoglobin and the homologous antibody but not other hemoglobin-antihemoglobin reactions. Clone JW151, which is known to contain a complete copy of the coding portion of the gamma globin mRNA, has been shown to produce a protein that reacts specifically with antibody to the chain of fetal hemoglobin, hemoglobin Kenya, and hemoglobin Bart's.
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