Open AccessIn vitro maturation of mesencephalic dopaminergic neurons from mouse embryos is enhanced in presence of their striatal target cells.
Author(s) -
Alain Prochiantz,
Umberto di Porzio,
Ann C. Kato,
Brigitte Berger,
J. Głowiński
Publication year - 1979
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.76.10.5387
Subject(s) - dopaminergic , catecholaminergic , dopamine , catecholaminergic cell groups , tyrosine hydroxylase , striatum , in vitro , biology , catecholamine , embryo , midbrain , microbiology and biotechnology , endocrinology , medicine , nigrostriatal pathway , substantia nigra , chemistry , neuroscience , central nervous system , biochemistry
Long-term survival of mesencephalic and striatal cells from mouse embryos in dissociated primary cultures is described. Catecholaminergic neurons in mesencephalic culutres were identified histochemically and by measuring [3H]dopamine uptake and synthesis from [3H]tyrosine. According to experiments using specific inhibitors of catecholamine uptake, at least two-thirds of the catecholaminergic neurons are dopaminergic. These neurons differentiated whether or not striatal target cells were present, but striatal cells stimulated the development of the dopaminergic neurons. [3H]Dopamine uptake was increased by at least 2-fold regardless of the age of the cocultures (4-15 days). Enhanced [3H]dopamine synthesis was also observed (at least 2-fold) at later times (12-15 days).