Open AccessCholecystokinin octapeptide-like immunoreactivity: histochemical localization in rat brain.
Author(s) -
Robert B. Innis,
Fernando M.A. Corrêa,
George R. Uhl,
B Schneider,
Solomon H. Snyder
Publication year - 1979
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.76.1.521
Subject(s) - cholecystokinin , immunohistochemistry , immunofluorescence , hippocampus , biology , pyramidal cell , medicine , endocrinology , hypothalamus , periaqueductal gray , cerebral cortex , chemistry , antibody , central nervous system , receptor , midbrain , immunology
Cholecystokinin octapeptide-like (CCK-OP-like) immunoreactivity was localized in the rat brain by using the indirect immunofluorescence method. Specificity in immunohistochemical studies was demonstrated by the virtual elimination of staining with either preimmune sera or sera preadsorbed with CCK-OP and by the achievement of similar fluorescent patterns with two different primary anti-CCK-OP sera. CCK-OP-like fluorescence was localized in neuronal cell bodies, fibers, and varicose terminals. The most dense collections of CCK-OP cells occurred in the periaqueductal gray and in the dorsomedial hypothalamus. Substantial numbers of cells and fibers also were present in the medial/dorsal and perirhinal cortex; more limited groups of cells were found in the pyramidal layer of the hippocampus and in the dorsal raphe.