Open AccessDifferences in intracellular distribution of plasminogen activator in growing, confluent, and transformed 3T3 cells.
Author(s) -
Susan Jaken,
Paul H. Black
Publication year - 1979
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.76.1.246
Subject(s) - intracellular , plasminogen activator , activator (genetics) , 3t3 cells , differential centrifugation , membrane , chemistry , microbiology and biotechnology , biophysics , biology , biochemistry , transfection , endocrinology , gene
The intracellular distribution of plasminogen activator in growing, confluent, and transformed 3T3 cells was investigated by using sucrose density gradient centrifugation. In confluent cells, which do not release plasminogen activator extracellularly, most of the activity was found in a heavy membrane fraction. In growing and transformed 3T3 cells, which do release plasminogen activator extracellularly in large amounts, most of the activity was found in lighter, plasma membrane-enriched fractions. A correlation between its presence in the light membrane fractions and the ability of the cells to release the plasminogen activator extracellularly is made.