Open AccessAlteration of myoblast phenotype by dimethyl sulfoxide.
Author(s) -
Armand F. Miranda,
E. Gerda Nette,
S. M. Mostafa Kamal Khan,
Kelvin G.M. Brockbank,
Michael Schonberg
Publication year - 1978
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.75.8.3826
Subject(s) - dimethyl sulfoxide , endoplasmic reticulum , myocyte , creatine kinase , chemistry , skeletal muscle , microbiology and biotechnology , extracellular , cell culture , fibroblast , biochemistry , biology , anatomy , in vitro , genetics , organic chemistry
Application of dimethyl sulfoxide to proliferating L8 myoblasts (an established cell line of rat skeletal muscle) for 72 hr completely prevented fusion and induction of creatine phosphokinase (EC 2.7.3.2) activity (an indicator of muscle differentiation). The growth pattern changed from the usual sheets of randomly oriented cells to flattened, whorled monolayers of elongated fibroblast-like cells. By electron microscopy, rough endoplasmic reticulum increased and extracellular material appeared that had the morphologic and staining characteristics of collagen. After 120 hr in dimethyl sulfoxide-containing medium, the cells secreted about 6 times more collagen than untreated controls. Dimethyl sulfoxide was ineffective when applied to L8 cells just prior to fusion, and effects of dimethyl sulfoxide were not readily reversible unless treated cells were subcultured at low density.