In vitro biosynthesis and chemical characterization of beta-lipotropin, gamma-lipotropin, and beta-endorphin in rat pars intermedia. | Zendy

Nabil G. Seidah | Zendy; Christina Gianoulakis | Zendy; Philippe Crine | Zendy; M. Lis | Zendy; Suzanne Benjannet | Zendy; R. Routhier | Zendy; Michel Chrètien | Zendy

Open Access

In vitro biosynthesis and chemical characterization of beta-lipotropin, gamma-lipotropin, and beta-endorphin in rat pars intermedia.

Author(s) -

Nabil G. Seidah,

Christina Gianoulakis,

Philippe Crine,

M. Lis,

Suzanne Benjannet,

R. Routhier,

Michel Chrètien

Publication year - 1978

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.75.7.3153

Subject(s) - pars intermedia , endorphins , biochemistry , biology , trypsin , beta (programming language) , peptide sequence , peptide , amino acid , in vitro , methionine , microbiology and biotechnology , endocrinology , medicine , pituitary gland , enzyme , hormone , computer science , gene , programming language

Three 3-hr incubations of pars intermedia cells from 40 rat pituitaries with [35S]methionine, [3H]lysine, and [3H]leucine sufficed for the identification and chemical characterization of biosynthesized beta-lipotropin, gamma-lipotropin, and beta-endorphin. From the molecular weight, migration on polyacrylamide gels, and sequence Met5, Lys9, Leu14,17, rat beta-endorphin was shown to be identical to its sheep homologue and no trace of Leu5 beta-endorphin could be detected. Rat beta-lipotropin differs from that of sheep in its elution properties on CM-cellulose, and its sequence shows Leu2,10,14, Lys20. Rat gamma-lipotropin shows the same NH2-terminal sequence as beta-lipotropin and is again different from its sheep homologue. The identification of rat beta-lipotropin was confirmed by its selective cleavage into beta-endorphin after trypsin digestion of the citraconylated peptide, a property not observed with rat gamma-lipotropin.

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