Open AccessATP-induced conformational changes in the restriction endonuclease from Escherichia coli K-12.
Author(s) -
Thomas A. Bickle,
Christine Brack,
Robert Yuan
Publication year - 1978
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.75.7.3099
Subject(s) - escherichia coli , atp hydrolysis , conformational change , dna , restriction enzyme , cleavage (geology) , endonuclease , biochemistry , nucleotide , enzyme , chemistry , escherichia coli proteins , adenosine triphosphate , hydrolysis , biology , gene , paleontology , atpase , fracture (geology)
ATP induces a conformational change in the Escherichia coli K-12 restriction enzyme that allows it to discriminate between unmodified and modified DNA recognition sequences. This conformational change does not require ATP hydrolysis. However, ATP hydrolysis is a requirement for DNA cleavage.