Open AccessGenetic recombination and complementation between bacteriophage T7 and cloned fragments of T7 DNA.
Author(s) -
Jim Campbell,
Charles C. Richardson,
F. William Studier
Publication year - 1978
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.75.5.2276
Subject(s) - bacteriophage , biology , in vitro recombination , complementation , plasmid , dna , genetics , escherichia coli , molecular cloning , microbiology and biotechnology , phagemid , gene , complementary dna , phenotype
Frafments of phage T7 DNA have been cloned in Escherichia coli by using the plasmid pMB9. Such cloned fragments are able to recombine with infecting phages, thus providing a means to integrate the physical and genetic maps of T7 DNA. Approximately 65% of the T7 DNA molecule has been found in clones so far, and analysis of these clones has mapped genes 12-17 with an accuracy of about 1% the total length of T7 DNA. At least some cloned segments can supply T7 functions to infecting phages.