z-logo
open-access-imgOpen Access
Kinetics of biphasic reconstitution of tobacco mosaic virus in vitro.
Author(s) -
Masahiro Fukuda,
Takeshi Ohno,
Yoshimi Okada,
Yoshiaki Otsuki,
Itaru Takebe
Publication year - 1978
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.75.4.1727
Subject(s) - rna , tobacco mosaic virus , rnase p , elongation , ribonuclease , infectivity , biophysics , kinetics , in vitro , biology , chemistry , virus , virology , biochemistry , materials science , physics , ultimate tensile strength , quantum mechanics , gene , metallurgy
The kinetics of the in vitro reconstitution of tobacco mosaic virus from its RNA and protein were studied by measuring the increase in turbidity, the development of ribonuclease-resistant infectivity, the emcapsidation of the terminal ends of the RNA, and the growth of rod length. The results showed that the reconstitution reaction consists of two processes in which the direction, timing, and rate of assembly are different. Rapid elongation of particles toward the 5' end of the RNA proceeds in the first 5-7 min to give intermediate particles of 260 nm in length in which only the 5' terminus of the RNA is encapsidated. The subsequent process requires 30-50 min, is accompanied by a slow increase in turbidity, and gives rise to rods of the full length, 300 nm. The 3' terminus becomes RNase resistant by this process with concomitant development of ribonuclease-resistant infectivity, showing that the 3'-distal portion of the RNA is encapsidated in the direction of 5' to 3'. The rate of rod elongation by the second process is less than 1/10 of that by the first process.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.
Having issues? You can contact us here