Open AccessStructure of psoralen-crosslinked ribosomal RNA from Drosophila melanogaster.
Author(s) -
Paul Wollenzien,
Douglas C. Youvan,
John E. Hearst
Publication year - 1978
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.75.4.1642
Subject(s) - rna , drosophila melanogaster , ribosomal rna , 18s ribosomal rna , biology , psoralen , microbiology and biotechnology , 5.8s ribosomal rna , ribosome , biophysics , genetics , dna , gene
Ribosomal RNA from Drosophila melanogaster photoreacted with hydroxymethyltrioxsalen has been examined by electron microscopy. Reproducible patterns of hairpins were found in both the 26S and 18S RNA. The frequency of these hairpins and the amount of incorporated drug were dependent upon the conditions under which the crosslinking was performed. A prominent central hairpin occurs in the 26S RNA and the break that interrupts the continuity of the RNA chain is located within it. In addition to several small hairpins, the crosslinked 18S RNA contains a large open loop.