Open AccessSpecies-dependent immunological differences between vertebrate brain tubulins.
Author(s) -
Janet L. Morgan,
Carter R. Holladay,
Brian S. Spooner
Publication year - 1978
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.75.3.1414
Subject(s) - antiserum , biology , heterologous , tubulin , radioimmunoassay , antigen , homologous chromosome , microbiology and biotechnology , structural similarity , epitope , microtubule , genetics , biochemistry , gene
The antigenic similarities and differences between highly purified brain tubulins from lamb, mouse, and chick embryo have been examined using rabbit antisera prepared against each of these tubulins. These antisera are capable of binding 125I-labeled tubulin in homologous or heterologous combinations, demonstrating immunological similarity between the tubulins. However, there are quantitative differences in the maximum amount of binding observed. Differences between the tubulins were further resolved by radioimmunoassays, comparing the ability of each of the tubulins to inhibit the binding of each 125I-labeled tubulin to each antiserum. Competition curves generated for all possible combinations revealed quantitative immunological differences between the tubulins that imply different densities of shared antigenic determinants on all three tubulins and a unique determinant on the chick tubulin molecule.