Open AccessDefective regulation of cholesterol biosynthesis and plasma membrane fluidity in a Chinese hamster ovary cell mutant.
Author(s) -
Michael Sinensky
Publication year - 1978
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.75.3.1247
Subject(s) - chinese hamster ovary cell , mutant , cholesterol , hamster , cell membrane , biology , cell , biosynthesis , biochemistry , microbiology and biotechnology , chemistry , endocrinology , enzyme , gene , receptor
A Chinese hamster ovary cell mutant resistant to killing by 25-hydroxycholesterol is shown to be defective in the regulation of cholesterol synthesis by exogenous cholesterol. When grown with various cholesterol supplements in delipidized serum, the mutant cell exhibits a variation in cholesterol content and plasma membrane fluidity that is not observed in the parental cell type.