Open AccessTwo thymidylate synthetases in Bacillus subtilis.
Author(s) -
Jan Neuhard,
A. R. Price,
Lise Schack,
Elisabeth Thomassen
Publication year - 1978
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.75.3.1194
Subject(s) - bacillus subtilis , thymidylate synthase , mutant , bacillaceae , biology , gene , bacillales , biochemistry , genetics , chemistry , bacteria , fluorouracil , chemotherapy
Bacillus subtilis grown at temperatures below 37 degrees contains two thymidylate synthetases, TSaseA and TSaseB. Their presence is dependent on functional thyA and thyB genes, respectively. When cells are grown at 46 degrees they only contain TSaseA activity. This allous an easy positive selection for thyA and thyA, THYB mutants. The two TSases have been physically separated, and they show similar overall requirements for activity. However, they differ significantly in both their kinetic and their physicochemical properties.