Open AccessPotato spindle tuber and citrus exocortis viroids undergo no major sequence changes during replication in two different hosts
Author(s) -
Elizabeth Dickson,
T.O. Diener,
Hugh D. Robertson
Publication year - 1978
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.75.2.951
Subject(s) - viroid , biology , lycopersicon , potato spindle tuber viroid , plant virus , rna , virology , botany , genetics , virus , gene
Potato spindle tuber viroid and citrus exocortis viroid, each purified from tomato (Lycopersicon esculentum ) and fromGynura aurantiaca , were iodinatedin vitro with125 I, digested with ribonuclease T1, and subjected to two-dimensional RNA fingerprinting analysis. With the exception of minor variations, each viroid retained its distinctive fingerprint pattern irrespective of the host species from which it was isolated. We conclude that the nucleotide sequences of these viroids are principally determined by the infecting viroid and not by the host.