Growth and maintenance of HeLa cells in serum-free medium supplemented with hormones. | Zendy

Sharon Hutchings | Zendy; Gordon Sato | Zendy

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Growth and maintenance of HeLa cells in serum-free medium supplemented with hormones.

Author(s) -

Sharon Hutchings,

Gordon Sato

Publication year - 1978

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.011

H-Index - 771

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.75.2.901

Subject(s) - transferrin , hela , chemically defined medium , epidermal growth factor , hormone , growth factor , biology , medicine , growth medium , endocrinology , growth hormone , aldosterone , cell culture , chemistry , biochemistry , cell , in vitro , genetics , receptor , bacteria

HeLa cells grow in a nutritionally complete synthetic medium (Ham's F12) supplemented with insulin, transferrin, hydrocortisone (aldosterone), fibroblast growth factor, and epidermal growth factor. This hormone-supplemented medium supports clonal growth, long-term cultivation, and a growth rate equal to that of serum-supplemented medium. The omission of any one of the five components results in less than maximal cell growth.

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