Open AccessIntervening sequence of DNA identified in the structural portion of a mouse beta-globin gene.
Author(s) -
S M Tilghman,
David C. Tiemeier,
J.G. Seidman,
B. Matija Peterlin,
M.H.F. Sullivan,
Jacob V. Maizel,
Philip Leder
Publication year - 1978
Publication title -
proceedings of the national academy of sciences
Language(s) - English
Resource type - Journals
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.75.2.725
Subject(s) - gene , biology , globin , coding region , genetics , nucleic acid sequence , microbiology and biotechnology , gene cluster , peptide sequence , sequence (biology) , dna , stop codon , base pair
The unusual electron microscopic appearance of a hybrid formed between 9S mouse beta-globin mRNA and its corresponding cloned gene segment is caused by at least one, and possibly two, intervening sequences of DNA that interrupt the mouse beta-globin gene. Such an interpretation is consistent with a paradoxical restriction site pattern previously noted in this gene and with the nucleotide sequence of that portion of the gene that spans both structural and intervening sequences. The large intervening sequence, approximately 550 base pairs in length, occurs in the structural globin sequence and immediately follows the beta-globin codon corresponding to amino acid 104. A smaller, putative intervening sequence is located close to the 5' end of the beta-globin-coding sequence but may reside beyond its initiation codon. The beta-globin gene thus appears to be encoded in two, and possibly three, discontinuous segments.
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