Open AccessApplication of higher derivative techniques to analysis of high-resolution thermal denaturation profiles of reassociated repetitive DNA.
Author(s) -
Richard E. Cuellar,
Glenn A. Ford,
Winslow R. Briggs,
William F. Thompson
Publication year - 1978
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.75.12.6026
Subject(s) - dna , resolution (logic) , chemistry , base pair , thermal stability , denaturation (fissile materials) , nucleic acid denaturation , derivative (finance) , analytical chemistry (journal) , chromatography , biochemistry , nuclear chemistry , base sequence , organic chemistry , artificial intelligence , computer science , financial economics , economics
We have analyzed high-resolution denaturation profiles of reassociated repetitive DNA sequences by using a combination of higher derivative analysis and curve-fitting techniques. Procedures originally used for resolution of components in complex absorption spectra were found to be applicable to high-resolution analysis of melting profiles of reassociated repetitive DNA sequences from pea DNA. Under conditions that eliminate the base composition effect on thermal stability (2.4 M tetraethylammonium chloride), such an anlysis can distinquish "thermal classes" of repetitive DNA duplexes exhibiting different amounts of base pair mismatch. Only a single thermal class is observed in reassociated Escherichia coli DNA whereas at least five classes can be reproducibly distinguished in pea and mung bean DNAs.