Open AccessCell-free synthesis and processing of the proteins of poliovirus.
Author(s) -
Ding S. Shih,
Chao-Yun T. Shih,
Olen M. Kew,
Mark A. Pallansch,
Roland R. Rueckert,
Paul Kaesberg
Publication year - 1978
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.75.12.5807
Subject(s) - capsid , poliovirus , hela , rna , protein biosynthesis , reticulocyte , cell free system , cell , microbiology and biotechnology , chemistry , biology , virus , biochemistry , virology , in vitro , gene
Poliovirus RNA can be translated completely and accurately in rabbit reticulocyte lysates; the nascent poly-protein is processed to give primary products 1a, X, and 1b indistinguishable from those made in poliovirus-infected HeLa cells. The capsid precursor protein 1a is processed to form the capsid proteins VP0, VP1, and VP3, while the noncapsid precursor 1b is processed to form protein 2.