Open AccessStructure of the orgin of DNA replication of bacteriophage fd.
Author(s) -
Christopher P. Gray,
Reinhold Sommer,
Christine Polke,
Ewald Beck,
Heinz Schaller
Publication year - 1978
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.75.1.50
Subject(s) - dna replication , dna , base pair , biology , heavy strand , dna polymerase ii , dna clamp , dna polymerase , microbiology and biotechnology , control of chromosome duplication , genetics , rna , gene , reverse transcriptase , transfer rna
An RNA-polymerase-protected DNA fragment of 125 nucleotides from the origin of single-strand to double-strand replication of bacteriophage fd (ori-DNA) was located on the physical map of the phage genome. A stretch of 187 base pairs of DNA including the ori-DNA was sequenced. This DNA segment contains regions with a highly asymmetric pyrimidine/purine distribution next to regions with 2-fold symmetry that form stable hairpin structures in the viral DNA strand.