Mutant RNA polymerase of Escherichia coli terminates transcription in strains making defective rho factor. | Zendy

L Guarente | Zendy; Jon Beckwith | Zendy

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Mutant RNA polymerase of Escherichia coli terminates transcription in strains making defective rho factor.

Author(s) -

L Guarente,

Jon Beckwith

Publication year - 1978

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.011

H-Index - 771

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.75.1.294

Subject(s) - rna polymerase , transcription (linguistics) , microbiology and biotechnology , rna dependent rna polymerase , polymerase , biology , operon , rna polymerase i , sigma factor , termination factor , mutant , antitermination , rna polymerase ii , rna , genetics , dna , promoter , gene , gene expression , linguistics , philosophy

We have isolated a rifampicin-resistant mutant of Escherichia coli RNA polymerase that restores transcription termination in strains with a defective rho protein. In such strains, the mutant RNA polymerase terminates transcription at normally rho-dependent sites at the end of the trp operon, in bacteriophage lambda, and within the lac operon. In addition, a strain with this mutant RNA polymerase remains viable with an amber mutation in rho, whereas a strain with wild-type RNA polymerase does not. These results suggest that the mutant RNA polymerase can terminate transcription at normally rho-dependent sites in the absence of rho.

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