Open AccessVariants of 3T3 cells lacking mitogenic response to epidermal growth factor.
Author(s) -
Rebecca M. Pruss,
Harvey R. Herschman
Publication year - 1977
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.74.9.3918
Subject(s) - epidermal growth factor , 3t3 cells , biology , colchicine , mitosis , microbiology and biotechnology , cell culture , epidermal growth factor receptor , receptor , cancer research , immunology , genetics , transfection
We have previously demonstrated that epidermal growth factor (EGF) can serve as a potent mitogen for 3T3 cells. We have now selected variant 3T3 cell lines unable to respond to EGF, in order to define cellular events unique to the EGF response and to distinguish which of these events are necessary and which are merely correlative to mitogenesis. By simultaneously treating cells with EGF and colchicine, we eliminated those cells stimulated by EGF to enter mitosis. Of the eight clonal EGF nonresponder variants selected by this procedure, none retains a functional EGF receptor. The EGF nonresponsive va-riant lines still retain the ability to respond to other mitogens.