Open AccessMorphological differentiation of neuroblastoma cells in medium supplemented with delipidated serum.
Author(s) -
Denis Monard,
Magda Rentsch,
Yvonne Schuerch-Rathgeb,
Ronald M. Lindsay
Publication year - 1977
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.74.9.3893
Subject(s) - neurite , oleic acid , neuroblastoma , fetus , biology , cellular differentiation , nerve growth factor , cell , cell culture , microbiology and biotechnology , biochemistry , chemistry , in vitro , pregnancy , receptor , gene , genetics
Neuroblastoma cells have been found to extend neurites when grown in a medium supplemented with delipidated fetal calf serum. Fetal calf sera from different commercial sources give rise to marked differences in the degree of this spontaneous morphological differentiation. The phenomenon can be prevented by the addition of certain fatty acids; oleic acid is especially effective. The serum-free conditioned medium from glial cells can quantitatively antagonize the effect of oleic acid, suggesting that glial factor activity could be due to components [lipids and/or macromolecular factors(s)] that are able to modify the properties of the neuroblastoma cell membrane.