Open AccessSeparation and infectivity of circular and linear forms of potato spindle tuber viroid.
Author(s) -
Robert A. Owens,
Eric F. Erbe,
A. Hadidi,
Russell L. Steere,
T.O. Diener
Publication year - 1977
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.74.9.3859
Subject(s) - potato spindle tuber viroid , formamide , viroid , infectivity , molecule , chemistry , linear molecular geometry , circular dna , circular rna , biology , rna , biochemistry , virology , virus , organic chemistry , genome , gene
Potato spindle tuber viroid can be separated into two fractions by polyacrylamide gelelectrophoresis in the presence of formamide and urea. One fraction contains predominantly circular molecules; the second fraction contains almost exclusively linear molecules. The purity of the fractions was estimated by electron microscopy of formaldehyde-denatured molecules. The length distributions of denatured circular and linear molecules were determined. Both circular and linear molecules were found to be infectious.