Packaging recombinant DNA molecules into bacteriophage particles in vitro. | Zendy

Barbara Höhn | Zendy; Kenneth Murray | Zendy

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Packaging recombinant DNA molecules into bacteriophage particles in vitro.

Author(s) -

Barbara Höhn,

Kenneth Murray

Publication year - 1977

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.011

H-Index - 771

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.74.8.3259

Subject(s) - recombinant dna , dna ligase , bacteriophage , dna , dna ligases , deoxyribonucleotide , in vitro recombination , microbiology and biotechnology , phagemid , in vitro , endogeny , biology , dna replication , chemistry , biochemistry , molecular cloning , oligonucleotide , escherichia coli , complementary dna , gene

Recombinant phage genomes made in reactions with purified enzymes may be recovered directly by packaging into phage heads in vitro. The process is efficient and nonselective and offers containment in initial stages of handling recombinant DNA. Ligase [poly(deoxyribonucleotide):poly-(deoxyribonucleotide) ligase (AMP-forming), EC 6.5.1.1] reaction products can recombine with endogenous phage DNA during packaging, but UV-irradiation eliminates the biological activity of the endogenous DNA.

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