Open AccessAuthentic beta-globin mRNA sequences in homozygous betaO-thalassemia.
Author(s) -
Gary F. Temple,
Judy C. Chang,
Yuet Wai Kan
Publication year - 1977
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.74.7.3047
Subject(s) - globin , microbiology and biotechnology , messenger rna , reticulocyte , complementary dna , biology , beta thalassemia , oligonucleotide , ribonuclease , beta (programming language) , hemoglobin , rna , thalassemia , gene , genetics , biochemistry , computer science , programming language
In a patient with homozygous betaO-thalassemia in whom studies of reticulocyte hemoglobin synthesis showed no beta-globin chain synthesis in vivo and in vitro, molecular hybridization studies revealed RNA sequences complementary to beta-globin cDNA. The fact that these sequences were authentic beta-globin mRNA was shown by fingerprint analysis of T1 ribonuclease-digested mRNA and by sequencing of oligonucleotides unique to beta-globin mRNA. The beta-mRNA that failed to direct beta-globin chain synthesis was not detectably shortened or degraded and contained poly(A) sequences.