Authentic beta-globin mRNA sequences in homozygous betaO-thalassemia. | Zendy

Gary F. Temple | Zendy; Judy C. Chang | Zendy; Y Kan | Zendy

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Authentic beta-globin mRNA sequences in homozygous betaO-thalassemia.

Author(s) -

Gary F. Temple,

Judy C. Chang,

Y Kan

Publication year - 1977

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.011

H-Index - 771

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.74.7.3047

Subject(s) - globin , microbiology and biotechnology , messenger rna , reticulocyte , complementary dna , biology , beta thalassemia , oligonucleotide , ribonuclease , beta (programming language) , hemoglobin , rna , thalassemia , gene , genetics , biochemistry , computer science , programming language

In a patient with homozygous betaO-thalassemia in whom studies of reticulocyte hemoglobin synthesis showed no beta-globin chain synthesis in vivo and in vitro, molecular hybridization studies revealed RNA sequences complementary to beta-globin cDNA. The fact that these sequences were authentic beta-globin mRNA was shown by fingerprint analysis of T1 ribonuclease-digested mRNA and by sequencing of oligonucleotides unique to beta-globin mRNA. The beta-mRNA that failed to direct beta-globin chain synthesis was not detectably shortened or degraded and contained poly(A) sequences.

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