Open AccessThermosensitive step associated with transfer of the Ti plasmid during conjugation: Possible relation to transformation in crown gall
Author(s) -
Jacques Tempé,
Annik Petit,
Marcella Holsters,
Marc Van Montagu,
Jeff Schell
Publication year - 1977
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.74.7.2848
Subject(s) - gall , agrobacterium tumefaciens , plasmid , transformation (genetics) , virulence , ti plasmid , strain (injury) , biology , agrobacterium , crown (dentistry) , microbiology and biotechnology , genetics , botany , gene , materials science , anatomy , composite material
It is reported here that transfer by means of a conjugative process of an oncogenic plasmid from a virulent strain of Agrobacterium tumefaciens to a strain of that organism that had been cured of the plasmid is thermosensitive. Since the thermosensitive step found in the conjugative process appears similar in every respect to a thermosensitive step that is involved in the transformation of a normal cell to a tumor cell in the crown gall disease of plants, it is suggested that the observed results may reflect the existence of a thermosensitive step that is common to these two processes.