Open AccessCryoenzymology in mixed solvents without cosolvent effects on enzyme specific activity.
Author(s) -
Pierre Douzou,
Claude Balny
Publication year - 1977
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.74.6.2297
Subject(s) - polyelectrolyte , chemistry , solvent , organic solvent , enzyme , sorting , component (thermodynamics) , enzyme catalysis , enzyme assay , organic chemistry , chemical engineering , thermodynamics , polymer , computer science , physics , engineering , programming language
Water-soluble polyelectrolytes in interaction with proteins are described. These polyelectrolytes make it possible to investigate enzyme-catalyzed reactions in cooled mixed solvents without the usual effects of their organic solvent component on enzyme specific activity. The applicability of techniques developed is illustrated by results obtained on several systems. The possibility of an electrostatic "sorting out" of solvents and its potentialities in cryoenzymology are discussed.