Open AccessInactivation and proteolytic cleavage of phage lambda repressor in vitro in an ATP-dependent reaction.
Author(s) -
Jeffrey W. Roberts,
Calvin W. Roberts,
David W. Mount
Publication year - 1977
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.74.6.2283
Subject(s) - repressor , lysogen , lambda phage , cleavage (geology) , bacteriophage , divalent , in vitro , biology , dna , microbiology and biotechnology , in vivo , biochemistry , chemistry , escherichia coli , gene , genetics , gene expression , paleontology , organic chemistry , fracture (geology)
We have reproduced in vitro the inactivation of bacteriophage lambda repressor that occurs in vivo when a lambda lysogen is treated with agents such as ultraviolet radiation that attack DNA. ATP and a divalent cation are required for the inactivation reaction. The ind- repressor is insensitive to the inactivation mechanism. A proteolytic cleavage of repressor accompanies inactivation in vitro, as it does in vivo.