Open AccessTranslation of melittin messenger RNA in vitro yields a product terminating with glutaminylglycine rather than with glutaminamide.
Author(s) -
Gerda Suchanek,
Günther Kreil
Publication year - 1977
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.74.3.975
Subject(s) - melittin , messenger rna , rna , translation (biology) , bee venom , chemistry , in vitro , biochemistry , biology , peptide , gene , zoology
Melittin messenger RNA from queen bee venom glands has been translated in a cell-free system from wheat germ. A product larger than promelittin is formed which has the carboxy-terminal sequence-Gln-Gln-GlyCOOH. Melittin and promelittin from venom glands terminate in -Gln-GlnCONH2. The possible role of the extra glycine residue in the formation of a COOH-terminal amide via a transamidase-like reaction is discussed.