Open AccessA nucleotide sequence from a ribonuclease III processing site in bacteriophage T7 RNA.
Author(s) -
Hugh D. Robertson,
Elizabeth Dickson,
John J. Dunn
Publication year - 1977
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.74.3.822
Subject(s) - rna , ribonuclease , endoribonuclease , biology , microbiology and biotechnology , ribonuclease iii , transcription (linguistics) , bacteriophage , nucleic acid sequence , messenger rna , rna editing , nucleotide , ribonuclease t1 , non coding rna , genetics , gene , escherichia coli , rnase p , linguistics , philosophy , rna interference
Transcription of that portion of the bacteriophage T7 genome encoding early functions yields RNA molecules about 7500 nucleotides long representing this entire early region. These long transcripts can be cleaved in vitro by highly purified Escherichia coli ribonuclease III (endoribonuclease III; EC 3.1.4.24), yielding five messenger RNAs identical to those produced in vivo. During this reaction, a small RNA fragment called F5 RNA is released, which is specified by the region of the T7 genome between genes 1.1 and 1.3. The following sequence of 32P-labeled F5 RNA has been determined using standard RNA sequencing techniques: pU-A-A-G-G-U-C-G-C-U-C-U-C-U-A-G-G-A-G-U-G-G-C-C-U-U-A-G-Uoh. The relative contributions of sequence and structure to ribonuclease III processing signals are considered in light of these findings.