Conditionally lethal ribosomal protein mutants: characterization of a locus required for modification of 50S subunit proteins. | Zendy

Sidney R. Kushner | Zendy; Valerie F. Maples | Zendy; W. Scott Champney | Zendy

Open Access

Conditionally lethal ribosomal protein mutants: characterization of a locus required for modification of 50S subunit proteins.

Author(s) -

Sidney R. Kushner,

Valerie F. Maples,

W. Scott Champney

Publication year - 1977

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.011

H-Index - 771

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.74.2.467

Subject(s) - ribosomal protein , 30s , mutant , ribosomal rna , biology , 50s , protein subunit , microbiology and biotechnology , locus (genetics) , eukaryotic large ribosomal subunit , 5s ribosomal rna , gene , ribosome , escherichia coli , genetics , rna , 18s ribosomal rna

Mutagenized P1 bacteriophage were used to transduce a marker (aroE) adjacent to the cluster or ribosomal protein genes located at 72 min on the Escherichia coli chromosome. Linked temperature-sensitive transductants were isolated and characterized. A mutant unable to grow at 44 degrees was found to be defective in protein synthesis both in vivo and in vitro. At the restrictive temperature mutant cells lost all polyribosomes. Analysis of the ribosomal proteins revealed alterations in at least four 50S subunit proteins. The mutation (called rimE1, ribosomal protein modification) mapped between rpsE and aroE. It is suggested that the rimE locus is the structural gene for an activity that modifies a selected number of ribosomal proteins.

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