Open AccessLoop structures in hybrids of early RNA and the separated strands of adenovirus DNA.
Author(s) -
Geoffrey R. Kitchingman,
Songjia Lai,
Heiner Westphal
Publication year - 1977
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.74.10.4392
Subject(s) - rna , dna , biology , adenovirus genome , gene , genome , nucleic acid thermodynamics , microbiology and biotechnology , gene expression , genetics , adenoviridae , genetic enhancement
Separated strands of adenovirus DNA were annealed with early cytoplasmic RNA and visualized in the electron microscope. DNA-RNA duplex regions within the DNA filaments could be recognized by their heavy contour. This contour was often interrupted at distinct locations by loops of displaced, single-stranded DNA. Loops have been observed and mapped in all four early regions of the genome. The structures appear to signal hitherto unknown mechanisms of eukaryotic gene expression.