Enzymatic synthesis of oligonucleotides of defined sequence: synthesis of a segment of yeast iso-1-cytochrome c gene. | Zendy

S Gillam | Zendy; Fritz Rottman | Zendy; Pat Jahnke | Zendy; Michael J. Smith | Zendy

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Enzymatic synthesis of oligonucleotides of defined sequence: synthesis of a segment of yeast iso-1-cytochrome c gene.

Author(s) -

S Gillam,

Fritz Rottman,

Pat Jahnke,

Michael J. Smith

Publication year - 1977

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.011

H-Index - 771

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.74.1.96

Subject(s) - oligonucleotide , phosphodiester bond , yeast , biochemistry , enzyme , polynucleotide phosphorylase , polynucleotide , primer (cosmetics) , cytochrome c , glycogen phosphorylase , sequence (biology) , chemistry , biology , gene , stereochemistry , microbiology and biotechnology , purine nucleoside phosphorylase , rna , organic chemistry , mitochondrion , purine

The deoxyribooligonucleotide, d(pT-T-A-G-C-A-G-A-A-C-C-G-G), constituting a segment of yeast iso-1-cytochrome c gene, has been synthesized by a combination of chemical and primarily enzymatic methods. The starting primer, d(pT-T-A-G1, was chemically synthesized by the phosphodiester method and was extended stepwise, by reactions catalyzed by polynucleotide phosphorylase.

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