Open AccessEnzymatic synthesis of oligonucleotides of defined sequence: synthesis of a segment of yeast iso-1-cytochrome c gene.
Author(s) -
Shirley Gillam,
Fritz M. Rottman,
Pat Jahnke,
Michael J. Smith
Publication year - 1977
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.74.1.96
Subject(s) - oligonucleotide , phosphodiester bond , yeast , biochemistry , enzyme , polynucleotide phosphorylase , polynucleotide , primer (cosmetics) , cytochrome c , glycogen phosphorylase , sequence (biology) , chemistry , biology , gene , stereochemistry , microbiology and biotechnology , purine nucleoside phosphorylase , rna , organic chemistry , mitochondrion , purine
The deoxyribooligonucleotide, d(pT-T-A-G-C-A-G-A-A-C-C-G-G), constituting a segment of yeast iso-1-cytochrome c gene, has been synthesized by a combination of chemical and primarily enzymatic methods. The starting primer, d(pT-T-A-G1, was chemically synthesized by the phosphodiester method and was extended stepwise, by reactions catalyzed by polynucleotide phosphorylase.