Open AccessNucleotide sequence of the operator-promoter region of the galactose operon of Escherichia coli.
Author(s) -
Richard E. Musso,
Roberto Di Lauro,
Martin Rosenberg,
Benoít de Crombrugghe
Publication year - 1977
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.74.1.106
Subject(s) - operon , gal operon , nucleic acid sequence , sequence (biology) , operator (biology) , escherichia coli , galactose , nucleotide , biology , lac operon , dna , genetics , promoter , transcription (linguistics) , microbiology and biotechnology , base pair , gene , biochemistry , transcription factor , repressor , gene expression , philosophy , linguistics
We have derived the nucleotide sequence of a segment of the operator-promoter region of the galactose operon of E. coli, by using a variety of DNA sequencing analyses. We have previously reported the sequence of the 5' terminal portion of gal mRNA [Musso, R. E., de Crombrugghe, B., Pastan, I., Sklar, J., Yot, P. & Weissman, S. (1974) Proc. Natl. Acad. Sci. USA 71, 4940-4944] and of the 59 base pairs preceding the startpoint of gal transcription (J. Sklar, S. Weissman, R. Musso, R. Di Lauro, & B. de Crombrugghe, submitted). In conjunction with those results, the present data provide the sequence of the gal operator-promoter region. This sequence is compared with similar sequences in other promoters and operators. Tentative mechanisms for the regulation of the galactose operon are discussed.