Open AccessDNA dependent synthesis of protein L12 from escherichia coli ribosomes, in vitro.
Author(s) -
Frederick K. Chu,
HsiangFu Kung,
Paul E. Caldwell,
Herbert Weissbach,
Nathan Brot
Publication year - 1976
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.73.9.3156
Subject(s) - ribosome , escherichia coli , guanosine , in vitro , ribosomal protein , dna , biochemistry , guanosine triphosphate , protein biosynthesis , chemistry , ribosomal rna , biology , microbiology and biotechnology , rna , gtp' , enzyme , gene
The in vitro synthesis of ribosomal protein L12 has been obtained in a coupled system with DNA extracted from the transducing phage lambdarifd18 as template. In addition, a second protein (molecular weight of 16,000) with immunological, chemical, and ribosome-binding characteristics similar to L12 is formed in this in vitro system. The synthesis of both proteins is depressed by one-half when guanosine-5'-diphosphate-3'-diphosphate is added to the reaction mixture.