Open AccessBiosynthesis of lipid-linked oligosaccharides and glycoprotein in aorta: stimulation by acceptor lipids isolated from liver.
Author(s) -
W. T. Forsee,
A.D. Elbein
Publication year - 1976
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.73.8.2574
Subject(s) - mannose , glycolipid , chemistry , biochemistry , oligosaccharide , glycoprotein , monosaccharide , hydrolysis , carbohydrate , acid hydrolysis
An acidic glycolipid fraction was isolated from porcine liver and partially purified on DEAE-cellulose. This material markedly stimulated the incorporation of mannose from GDP-[14C]mannose into lipid-linked oligosaccharides and into glycoprotein, but did not stimulate incorporation into the mannosyl-phosphoryl-polyprenol. The products formed from GDP-[14C]mannose in the presence of acceptor lipid were a series of lipid-linked oligosaccharides of mannose and N-acetylglucosamine (GlcNAc) which differ from each other in the size of the oligosaccharide moieties. The acceptor lipid fraction was partially characterized in terms of its carbohydrate composition by mild acid hydrolysis and reduction with NaB3H4. The [3H]sugars were then separated into a number of monosaccharides and oligosaccharides by paper chromatography. Strong acid hydrolysis of two of the larger oligosaccharides released from the acceptor lipid followed by reduction with NaB3H4 gave [3H]mannitol and [3H]GlcN-ol in ratios of 2.8:2 and 3.8:2, indicating that these compounds probably were penta- and hexasaccharides. In addition, GlcNAc was shown to be at the reducing terminus and alpha-linked mannose at the nonreducing ends of the molecules. The results suggest that the acceptor lipid fraction is composed of oligosaccharides of (Man)n leads to GlcNAc leads to GlcNAc, probably attached to a polyprenol through a pyrophosphoryl linkage.