Open AccessFusion of protoplasts of Bacillus megaterium.
Author(s) -
Katalin Fodor,
L Alföldi
Publication year - 1976
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.73.6.2147
Subject(s) - bacillus megaterium , protoplast , polyethylene glycol , recombinant dna , bacteria , biology , microbiology and biotechnology , bacillus (shape) , bacillaceae , bacillales , calcium , bacteriophage , biochemistry , chemistry , gene , genetics , escherichia coli , bacillus subtilis , organic chemistry
Protoplasts of two doubly auxotrophis strains of Bacillus megaterium were fused by nascent calcium phosphate or by polyethylene glycol treatment, and then allowed to revert to bacillary form on selection media. The colonies formed were analyzed and found to be mixed populations of individual bacteria apparently containing parental, recombinant, and segregating genotypes. The evidence suggested that segregation was transitory, lasting only for a few clonings and reisolations.