Effect of Xenopus laevis oocyte extract on supercoiled simian virus 40 DNA: formation of complex DNA.
Author(s) -
Domenica Gandini Attardi,
G. Martini,
Emilio Mattoccia,
Glauco P. TocchiniValentini
Publication year - 1976
Publication title -
proceedings of the national academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.73.2.554
Subject(s) - xenopus , dna , dna supercoil , simian , biology , oocyte , base pair , virus , dna clamp , microbiology and biotechnology , biophysics , virology , chemistry , biochemistry , embryo , dna replication , rna , reverse transcriptase , gene
A soluble cell-free extract derived from stage 6 Xenopus laevis oocytes is described. From supercoiled simian virus 40 DNA the extract produces nicked circles (having a single-strand scission), linear molecules of full unit size, shorter length fragments, and various forms of complex DNA.
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