A method for determination of the in situ distribution of chromosomal proteins. | Zendy

Lee M. Silver | Zendy; Sarah C. R. Elgin | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

A method for determination of the in situ distribution of chromosomal proteins.

Author(s) -

Lee M. Silver,

Sarah C. R. Elgin

Publication year - 1976

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.011

H-Index - 771

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.73.2.423

Subject(s) - polytene chromosome , chromatin , in situ , biology , chromosome , staining , karyotype , fixation (population genetics) , chromosomal analysis , microbiology and biotechnology , genetics , dna , gene , chemistry , organic chemistry

A technique has been developed for "staining" cytological preparations by indirect immunofluorescent methods that permits determination of the in situ distribution of chromosomal proteins. The method is particularly oriented to the use of polytene chromosome squashes from Drosophila salivary glands. Control experiments indicate that the fixation methods used allow little or no extraction or rearrangement of the chromosomal proteins. The results obtained demonstrate the specific in vivo chromosomal locations of nonhistone proteins purified from isolated chromatin. The technique is apparently capable of resolution at the level of the chromomere or band, the unit of genetic organization in Drosophila.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research