Open AccessSynthesis of Rauscher murine leukemia virus-specific polypeptides in vitro.
Author(s) -
A. L. J. Gielkens,
D. van Zaane,
Henri P.J. Bloemers,
H. Bloemendal
Publication year - 1976
Publication title -
proceedings of the national academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.73.2.356
Subject(s) - reticulocyte , polysome , murine leukemia virus , lysis , rna , biology , in vitro , messenger rna , virus , microbiology and biotechnology , biochemistry , protein biosynthesis , molecular mass , leukemia , virology , ribosome , gene , enzyme , immunology
The biosynthesis of specific polypeptides directed by purified viral messenger RNA from JLS-V9 cells infected with Rauscher leukemia virus has been studied in a rabbit reticulocyte lysate. The 35S viral mRNA gives rise to two major products of 65,000 and 72,000 molecular weight. The synthesis of specific polypeptides was also investigated in lysates derived from infected cells. The main products were polypeptides with molecular weights of 65,000, 76,000, and 82,000, and were preferentially made in association with membranes. The relative content of the virus-specific polypeptide of 65,000 molecular weight, synthesized in a cell-free system supplemented with purified polyribosomes, is considerably higher for membrane-bound polyribosomes.
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