Open AccessTubulin requires tau for growth onto microtubule initiating sites.
Author(s) -
George B. Witman,
Don W. Cleveland,
Murray D. Weingarten,
Marc W. Kirschner
Publication year - 1976
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.73.11.4070
Subject(s) - microtubule , tubulin , microtubule associated protein , microbiology and biotechnology , biophysics , microtubule nucleation , biology , chemistry , biochemistry , cell , centrosome , cell cycle
Tubulin purified by phosphocellulose chromatography and free of accessory proteins will not form microtubules in the absence or presence of microtubule initiating sites (flagellar microtubules). The capacity for growth onto pre-existing "seeds" can be restored by the addition of small quantities of partially purified tau protein. Larger quantities restore the capacity for spontaneous assembly. These results suggest that tubulin requires tau for both initiation and growth of microtubules and that tau is incorporated into the microtubule throughout its length.