Open AccessDNA gyrase: an enzyme that introduces superhelical turns into DNA.
Author(s) -
Martin Gellert,
Kiyoshi Mizuuchi,
Mary H. O’Dea,
Howard A. Nash
Publication year - 1976
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.73.11.3872
Subject(s) - dna supercoil , dna gyrase , superhelix , circular bacterial chromosome , dna , replisome , escherichia coli , biology , dna clamp , enzyme , in vitro recombination , microbiology and biotechnology , dna replication , biochemistry , molecular cloning , gene , reverse transcriptase , rna , peptide sequence
Relaxed closed-circular DNA is converted to negatively supercoiled DNA by DNA gyrase. This enzyme has been purified from Escherichia coli cells. The reaction requires ATP and Mg++ and is stimulated by spermidine. The enzyme acts equally well on relaxed closed-circular colicin E1, phage lambda, and simian virus 40 DNA. The final superhelix density of the DNA can be considerably greater than that found in intracellularly supercoiled DNA.