A model for compartmentation of de novo and salvage thymidine nucleotide pools in mammalian cells. | Zendy

Daniel Kuebbing | Zendy; Rudolf Werner | Zendy

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A model for compartmentation of de novo and salvage thymidine nucleotide pools in mammalian cells.

Author(s) -

Daniel Kuebbing,

Rudolf Werner

Publication year - 1975

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.011

H-Index - 771

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.72.9.3333

Subject(s) - thymidine , nucleotide , nucleotide salvage , hela , dna synthesis , biology , biochemistry , dna , microbiology and biotechnology , de novo synthesis , chemistry , cell , gene , enzyme

When [3H]thymidine is added to HeLa cells grown in thymidine-free medium it is incorporated into DNA almost immediately at full specific activity, blocking any further incorporation of de novo synthesized thymidine nucleotides. Apparently, de novo nucleotide pools [Baumunk, C.N. & Friedman, D.L. (1971) Cancer Res. 31, 1930--35] and salvage nucleotide pools are compartmentalized in HeLa cells. After one generation in the presence of thymidine, the cells begin utilizing nucleotides from both pools to about equal extent.

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